CELVIVO BIOARRAY MATRIX DRIVE

Our cells are grown using the clinostat principle. We form three-dimensional spheroids, and then we incubate them for weeks until they become functional “synthetic organs” (see video below)

We create microsize spheroids and then we incubate them in the CelVivo bioreactor. After 20 days, the cells stop proliferating and acquire functional properties close to original solid tissues. This way we can produce more accurate models of living tissues in a cell lab.

By growing the same liver cell line (HepG2/C3A) as functional spheroids or canonical flat culture, we obtain dramatic differences in the specific proteome. 3D cells create cell-cell communications and stop proliferating at rapid pace, leading to more efficient translation of proteins specific of liver function (e.g. metabolism, metal binders, response to metabolic stress). Flat cultures are more busy producing new DNA, RNA and proteins typical of unspecific cell proliferation.

Proteins enriched in 3D are color coded in red, 2D in blue.