Current grants as principal investigators
This is a list of currently active funding that sponsor the Sidoli lab in various aspects of their research. A big thank you to those who support our science!
Einstein-Mount Sinai Diabetes Research Center
Title: Deciphering the role of the metabolically-induced histone modification H3K27ac in Heart Failure with Preserved Ejection Fraction (HFpEF) syndrome in patient samples & a new mouse model
Major Goals: Analyze chromatin dynamics of HFpEF heart patient samples to link metabolism with gene regulation.
Merck
Title: High-Throughput Peptide Mapping for ADC Reaction Monitoring
Major Goals: Develop an assay to do rapid analysis of modified antibodies
Relay Therapeutics
Title: Protein oxidation automation
Major Goals: Develop a new workflow to identify protein conformation via oxidation foot printing.
Xseed award (Deerfield)
Title: A proteomics approach to identify effective drug combinations for the treatment of K. pneumoniae
Major Goals: Develop an assay based on MALDI-MS to identify Klebsiella resistance in Montefiore patients
Awards to the lab
2022 Eastern Analytical Symposium Young Investigator Award
Simone receives the 2022 Eastern Analytical Symposium Young Investigator Award. The symposium takes place every year in New Jersey, and Simone will participate as invited speaker.
Einstein-Montefiore SARS-CoV-2 microgrants
Einstein-Montefiore sponsors the lab for a SARS-CoV-2 (COVID-19) research project. We will use this funding to investigate the potential of exhaled breath condensate in infection diagnostics.
NAM Healthy Longevity Grand Challenge Catalyst award
The Japan Agency for Medical Research and Development has kindly awarded to the labs of Sidoli, Arioka and Basu new funding to continue their project in epigenetics and mental disorder. The project aims to improve healthspan and lifespan of patients affected by schizophrenia and related conditions.
Einstein Nathan Shock Center of Excellence
Thank you to the Einstein Nathan Shock Center of Excellence for supporting us with funding for aging research! The Sidoli lab received funding to study a specific protein modification on the chromatin of centenarians.
Interstellar initiative
The New York Academy of Sciences (NYAS) and the Japan Agency for Medical Research and Development (AMED) developed a platform for the world’s most promising Early Career Investigators to connect with peers. Simone has been selected to participate to their 2019-2020 workshop, where significant funding is provided to the teams for establishing collaborations.
Umberto Mortari award
Merck/MSD and Fortune Italia sponsor an award for one Italian scientist <40 who has returned to Italy and one who has remained abroad and runs successful science outside of Italy. The first recipients of these two awards are Velia Siciliano (IIT) and Simone Sidoli, respectively. A special thank you goes to Alfonso Bellacosa (Fox Chase Cancer Center) for proposing Simone’s candidacy.
Collaborative grants
A special thank you to our collaborators who share with us part of their funding to work together towards completing these important research programs!
1R21AI174037 (PI: Chandran)
Title: Comprehensive genetic dissection of poxvirus membrane assembly and function
Major Goals: Genetic and biochemical studies of how infectious poxvirus particles are assembled and released from cells.
R01 AI153008 01A1 (PI: Prasad)
Title: CCL2-CCR2b Signaling in HIV-1 Fitness and Disease: Role of Host Genetic Polymorphisms
Major Goals: (1) Dissect the mechanism by which CCL2 mobilizes ALIX for use by HIV-1 Gag for viral budding, (2) examine the effect of CCR2bV64I polymorphism on CCL2 signaling and HIV replication fitness, and (3) examine the effect of CCL2 2518G polymorphism on CCL2 signaling and HIV replication fitness
W81xWH-21-1-0207 (BC200410P1) (PI: Guo)
Title: Investigating how tumor suppressor MLL3 mutations promote tumor infiltrating regulatory T and B cells
Major Goals: Exploring the role of MLL3 (KMT2C) mutation in modulating tumor immune microenvironment
2R01AI124753-07A1 (PI: Weiss)
Title: Microsporidia: invasion apparatus
Major Goals: Understanding the mechanism of invasion and the structural biology and composition of the polar tube of microsporidia. Microsporidia are organisms related to the Fungi, causing infections in humans.
1 R21 AI166194-01 (PI: Kielian)
Title: Identification and characterization of host proteins involved in the alphavirus exit pathway
Major Goals: Use a novel capsid protein pull-down strategy and mass spectrometry to identify capsid associated host proteins, and to define the role of these candidates in the alphavirus exit pathway.
2 R01 HL069438-21 (PI: Kelly)
Title: In vivo mechanisms mediating sickle cell vaso-occlusion and organ damage
Major Goals: Identify the mechanistic contributions of aged neutrophils, the specific bacterial strains that protect or promote the disease, and their interplay with nerve-derived signals.
1U01HG012051-01 (PI: Huangfu)
Title: Genomic control of gene regulatory networks governing early human lineage decisions
Major Goals: Perform chromatin immunoprecipitation followed by mass spectrometry of transcription factors involved in early embryogenesis.
5R21AI155603-02 (PI: Bzik)
Title: Glucosylation Regulates Cyst Wall Formation, Stability, and Persistence of the AIDS Pathogen Toxoplasma gondii
Major Goals: proteomic analysis of Toxoplasma gondii samples to identify sWGA binding proteins.
1 R01 GM111752 (PI: Gu)
Title: RNA-mediated Chromatin Regulation and Epigenetic Inheritance in C. elegans
Major Goals: Identify the key regulators of C. elegans heterochromatin
1R01AI164864-01A1 (PI: Daily)
Title: Defining the mechanism of coma in cerebral malaria
Major Goals: This project will study the role of pipecolic acid in the etiology of cerebral malaria coma, a condition that contributes to worldwide morbidity and mortality and secondly, determine how it is produced by the malaria parasite.
1R01HL164772-01 (PI: Kitsis)
Title: Caspase-9 as a nodal point connecting necrotic and apoptotic cell death in myocardial infarction
Major Goals: This project delineates key aspects of the pathway and investigates whether its therapeutic inhibition could provide the basis for a new therapy to limit cardiac damage during myocardial infarction.
1R01GM146409-01 (PI: Morey/Verdun)
Title: Altering the chromostasis and genome stability by modulating histone methylation
Major Goals: Establish the epigenetics and DNA repair mechanisms affected by the expression of a mutant histone 3 (H3K36M) in human cells.
Completed grants
Finally, here is a list of funding that previously supported the lab. We did not want to forget to acknowledge those who have been instrumental in elevating our group towards the years.
American Federation for Aging Research, Sagol network (PI: Sidoli)
Title: Accessible heterochromatin in exceptional longevity, a proteomics signature
Major Goals: Quantify hybrid histone codes in the chromatin of centenarians, proteins associated to decondensed chromatin and validate the role of EXOSC4 in maintaining chromatin homeostasis.
5 R01GM129350-04 (PI: Brenowitz)
Title: How MECP2 discriminates epigenetic marks is still a mystery
Major Goals: Our proposed studies of the methyl-CpG- binding protein 2 (MeCP2) explore the mechanisms by which this regulatory protein competes with linker histone proteins for chromatin DNA and discriminates sites of epigenetic modification
1S10OD030286-01 (PI: Sidoli)
Title: Orbitrap Exploris 480 Basic System
Major Goals: Shared instrument grant to support the Einstein Proteomics Core.
Leukemia Research Foundation (PI: Sidoli)
Title: Accessible heterochromatin as new target against AML development
Major Goals: Identifying the readers of conditionally accessible heterochromatin in AML genotypes with mutations in chromatin remodelers
Basic Biology of Aging 2020 (PI: Sidoli)
Title: Identifying markers of accessible heterochromatin in centenarians to understand enhanced healthspan
Major Goals: (1) Identify proteins that specifically bind to accessible heterochromatin in B-cells of centenarians and their progeny. (2) Identify specific readers of histone succinylation.
NAM Healthy Longevity (PI: Sidoli, Arioka, Basu)
Title: Histone modifications as new targets for pathophysiology of accelerated brain aging associated with mental disorders: Application for development of treatment and diagnostic method
Major Goals: Three-lab collaboration in investigating chromatin dynamics in mental disorders
Interstellar initiative (PI: Sidoli, Arioka, Basu)
Title: Schizophrenia towards next-generation therapy
Major Goals: Three-lab collaboration in investigating chromatin dynamics in mental disorders
P30 CA013330 47 (PI: Goldman)
Title: Einstein Cancer Center Core grant
Major Goals: Providing the Einstein Cancer Center with proteomics services